Optimization of the Zebrafish Larvae Pentylenetetrazol-Induced Seizure Model for the Study of Caffeine and Topiramate Interactions

Epilepsy is a common neurological disorder characterized by seizures that cause neurobiological and behavioral impairment. Caffeine (CAF), which the most widely consumed stimulant in world, reported to influence epileptic antiepileptic drugs, especially topiramate (TPM). The aim of study was optimize zebrafish larvae pentylenetetrazol-induced seizure model for CAF TPM interactions, include determination dose space, delivery an analytical method monitoring CAF, TPM, metabolite paraxanthine (PAR) Zebrafish larvae. Methods: larvae, 4 days post-fertilization, were incubated 18 h with or + subsequent locomotor activity assessment. Seizures evoked adding PTZ solution obtain final concentration 20 mM. Subsequently, liquid chromatography–mass spectrometry (LC–MS/MS) used simultaneously assess levels both (50 mg/L) (75 μM) given separately decreased average compared group but, however, not able lower it control level. Co-administration 25 mg/L 50 μM suppressed same Adding decrease measured level Until proven otherwise, consumption should be regarded as potential determinant modulation TPM’s efficacy management seizures. optimized will contribute standardization studying interactions building understanding molecular bases interaction.